![]() After gel electrophoresis, the proteins were transferred using the method and conditions shown above. Local background corrected volume per lysate was plotted for each lane. Invitrogen iBright FL1500 Imaging System was used for image capture. Thermo Scientific SuperSignal West Dura chemiluminescent substrate was used for detection. Primary antibody was removed and GAM-HRP secondary antibody (1:120,000 in TBST) was added to blots and incubated for 2 hours. ![]() Hsp70 primary antibody (1:1,000 in clear milk) was added to blots and incubated for 12 hours at room temperature. Blots were incubated with Thermo Scientific Pierce Clear Milk Blocking Buffer for 30 minutes. Immunoprocessing was completed using the Invitrogen Bandmate Automated Western Blot Processor. The instrument is also effective for standard 30- to 60-minute semi-dry transfer protocols based on traditional Towbin buffer.The iBlot 3 system demonstrates better transfer efficiency. The Power Blotter System achieves highly efficient transfer in seven minutes when used with Power Blotter Select Transfer Stacks or ten minutes with Power Blotter 1-Step Transfer Buffer. The easy-touch programming feature allows custom transfer settings to be quickly created, saved, and run. The included Power Blotter Station has an easy-to-use color LCD touchscreen interface with pre-programmed transfer methods for different numbers and sizes of gels and ranges of molecular weight proteins. The Power Blotter XL Cassette, included in the Power Blotter XL System, enables the simultaneous transfer of up to four mini-size or two midi-size gels. Learn more about the semi-dry transfer device: Power Blotter XL System Versatile-compatible with Power Blotter Select Transfer Stacks and Pierce 1-Step Transfer Buffer for rapid blotting programs or Towbin transfer buffer for conventional semi-dry transfer methods.Flexible-simultaneous transfer of 1 to 4 mini-size or 1 to 2 midi-size gels with the Power Blotter Cassette XL.Easy-touch programming-convenient pre-programmed transfer methods or create, save, and run customized transfer methods.Integrated power supply-seamless operation between control unit and cassette no need for external power supply.Fast-5- to 12-minute transfer when used with Power Blotter Select Transfer Stacks or Power Blotter 1-Step Transfer Buffer.Efficient-high transfer efficiency with a broad range of protein sizes compared to conventional semi-dry or wet (tank) transfer methods.The Power Blotter System includes an integrated power supply optimized to enable consistent and high-efficiency protein transfer.įeatures of the Power Blotter XL System include: The Invitrogen Power Blotter XL System is specifically designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes in 5 to 12 minutes.
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